Expression of P1 DNA in mammalian cells and transgenic mice
β Scribed by Sally P.A. McCormick; Macrae F. Linton; Stephen G. Young
- Book ID
- 113409617
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 588 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1050-3862
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## Abstract ## Background Supercoiled topology of transfected plasmid DNA (pDNA) is critical for transgene expression in mammalian cells. In the present study, we analysed transgene expression of transfected supercoiled pDNA concatemers. ## Methods Jurkat T cells were transfected with a supercoi
We read with interest the article by Maucksch et al. [1], who analysed transgene expression of transfected supercoiled 4.7 kb monomeric and 9.4 kb dimeric plasmid concatemer in Jurkat T cells using electroporation. The authors reported that the relative number of electrotransferred gene copies per n