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Expression of P1 DNA in mammalian cells and transgenic mice

✍ Scribed by Sally P.A. McCormick; Macrae F. Linton; Stephen G. Young


Book ID
113409617
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
588 KB
Volume
11
Category
Article
ISSN
1050-3862

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## Abstract ## Background Supercoiled topology of transfected plasmid DNA (pDNA) is critical for transgene expression in mammalian cells. In the present study, we analysed transgene expression of transfected supercoiled pDNA concatemers. ## Methods Jurkat T cells were transfected with a supercoi

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We read with interest the article by Maucksch et al. [1], who analysed transgene expression of transfected supercoiled 4.7 kb monomeric and 9.4 kb dimeric plasmid concatemer in Jurkat T cells using electroporation. The authors reported that the relative number of electrotransferred gene copies per n