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Bifidobacterium suppresses IgE-mediated degranulation of rat basophilic leukemia (RBL-2H3) cells

✍ Scribed by Gaku Harata; Fang He; Kyoko Takahashi; Akira Hosono; Manabu Kawase; Akira Kubota; Masaru Hiramatsu; Shuichi Kaminogawa

Book ID
108578380
Publisher
Center for Academic Publications, Japan
Year
2010
Tongue
English
Weight
134 KB
Volume
54
Category
Article
ISSN
0385-5600

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Simultaneous determination of intracellular calcium concentration and histamine secretion in rat basophilic leukemia cells (RBL-2H3)
✍ Kazutaka Maeyama; Masayuki Sasaki; Takehiko Watanabe πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 874 KB

In rat basophilic leukemia cells (RBL-2H3), a tumor analogue of mast cells, the aggregation of IgE receptors initiates increase in the intracellular concentration of calcium ([Ca2+]i), monitored with the fluorescent Ca probe fura-2, and finally results in histamine secretion. In cell suspensions, ho