Enhancement of the release of inflammatory mediators by substance P in rat basophilic leukemia RBL-2H3 cells
β Scribed by Ben-Ching Liao; Rolis Chien-Wei Hou; Jen-Shu Wang; Kee-Ching G. Jeng
- Publisher
- BioMed Central
- Year
- 2006
- Tongue
- English
- Weight
- 281 KB
- Volume
- 13
- Category
- Article
- ISSN
- 1021-7770
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π SIMILAR VOLUMES
In rat basophilic leukemia cells (RBL-2H3), a tumor analogue of mast cells, the aggregation of IgE receptors initiates increase in the intracellular concentration of calcium ([Ca2+]i), monitored with the fluorescent Ca probe fura-2, and finally results in histamine secretion. In cell suspensions, ho
To detect low levels of histamine, we developed a histamine microsensor using recombinant histamine oxidase. Histamine oxidase with a histidine tag was readily purified using a histidine affinity column. The enzyme showed higher catalytic activity on histamine than diamines (e.g., putrescine and cad