Abstract
Axonal transport of the 16S molecular form of acetylcholinesterase (16SβAChE) in doubly ligated rat sciatic nerves was studied by means of velocity sedimentation analysis on sucrose gradients. This form of AChE was selectively confined to motor, and not to sensory, fibers in the sciatic nerve, where it represented 3β4% of total AChE. Its activity increased linearly with time (4β20 hr) in nerve segments (7 mm) proximal to the central ligature (4.5 mU/24 hr) and distal to the peripheral ligature (2.0 mU/24 hr). From the linear rates of accumulation of 16SβAChE, we conclude that the enzyme is conveyed by anterograde and retrograde axonal transport at velocities close to those previously defined for the movement of total AChE (410 mm/day, anterograde; 220 mm/day, retrograde). The transport of AChE molecular forms, other than the 16S form, could not be resolved presumably due to their presence in blood as well as at extraaxonal sites. The present findings are consistent with the view that in rat sciatic nerve most, if not all, of the small portion of total AChE (approximately 3%) which is transported may be accounted for by 16SβAChE.