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Axonal transport of glycerophospholipids in regenerating sciatic nerve of the rat during aging

✍ Scribed by Dr. Mario Alberghina; M. Viola; F. Moschella; A. M. Giuffrida

Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
482 KB
Volume
9
Category
Article
ISSN
0360-4012

No coin nor oath required. For personal study only.

✦ Synopsis

The effect of age upon the axoplasmic transport of glycerophospholipids has been studied using as a model the regenerating sciatic nerve of young (2-month-old), young adult (6-month-old), middle-aged (16-month-old), and aged (20-month-old) male rats. The right sciatic nerve was crushed 0.5 mm down the incisura ischiadica. Four and nine days after the lesion, a mixture of [2-3H] glycerol and [meth~l-'~C] choline was bilaterally injected into the spinal cord, at a level of the L4-L5 vertebrae. The animals were killed 18 hr after the isotope injection. Proximal and distal portions of crushed nerve and of contralateral sham-operated ones were dissected and consecutive 5-mm segments were subjected to lipid extraction and analysis.

The findings of the present study are summarized as follows:

(1) The accumulation of labeled lipid material axonally transported four days after nerve injury was mainly located at the crush site in young, young adult, middle-aged, and aged rats. The accumulation of both 3H-glycerolipids and I4C-choline phospholipids in postcrush segments was markedly higher for young and young adult than for aged rats, four and nine days after crush; (2) the average rate of axonal regeneration, determined between days 4 and 9 following crush injury was 3.6 and 4.2 mmfday for 2-month-old and 6month-old rats, respectively; it decreased to the value of 2.5 mmfday for 16-20month-old rats.

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