𝔖 Bobbio Scriptorium
✦   LIBER   ✦

“BEST” Homonuclear Adiabatic Decoupling for 13C- and 15N-Double-Labeled Proteins

✍ Scribed by Shanmin Zhang; David G. Gorenstein

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
86 KB
Volume
138
Category
Article
ISSN
1090-7807

No coin nor oath required. For personal study only.

✦ Synopsis

The cyclic irradiation sidebands appearing in homonuclear adiabatic decoupling are calculated in detail, which reveals the origin of the antisymmetric sidebands. The sidebands can be inverted by inserting an initial decoupling with a different period, but the same f 1rms as the main decoupling that is required for Bloch-Siegert shift compensation. The sidebands can be eliminated in a broad decoupling range by adding spectra of opposite sidebands. Based on this scheme, an offset-independent double-adiabatic decoupling, named Bloch-Siegert Shift Eliminated and Cyclic Sideband Trimmed Double-Adiabatic Decoupling, or "BEST" decoupling for short, is constructed, which not only compensates the Bloch-Siegert shift as shown earlier by Zhang and Gorenstein (1998) but also eliminates residual sidebands effectively.

📜 SIMILAR VOLUMES

Bloch–Siegert Shift Compensated and Cycl
Bloch–Siegert Shift Compensated and Cyclic Irradiation Sidebands Eliminated, Double-Adiabatic Homonuclear Decoupling for13C- and15N-Double-Labeled Proteins
✍ Shanmin Zhang; David G. Gorenstein 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 194 KB

A Gaussian-shaped, offset-independent adiabatic decoupling is adopted to decouple 13CO from 13C alpha or vice versa for 13C- and 15N-double-labeled proteins, together with a compensating decoupling applied on the opposite side of the 13C alpha resonance frequency. In a quite broad range, the double-

“Double-WURST” Decoupling for15N- and13C
“Double-WURST” Decoupling for15N- and13C-Double-Labeled Proteins in a High Magnetic Field
✍ Shanmin Zhang; Jian Wu; David G. Gorenstein 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 204 KB

A ''Double-WURST'' decoupling sequence is constructed for sitogether with suitable phase cycles, mainly to compensate multaneously decoupling the 13 CO and 13 C a regions in 15 N/ 13 C-doupulse imperfections. ## ble-labeled proteins. It uses frequency-shifted WURST decoupling The early design of

Sequence-Specific Assignment of Aromatic
Sequence-Specific Assignment of Aromatic Resonances of Uniformly 13C,15N-Labeled Proteins by Using 13C- and 15N-Edited NOESY Spectra
✍ Zhi Lin; Yingqi Xu; Shuai Yang; Daiwen Yang 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 282 KB 👁 1 views

The precision of a protein structure determined by NMR spectroscopy is strongly influenced by the number of longrange NOE interactions per residue. Aromatic and methylcontaining residues are frequently located in the hydrophobic interior of proteins and yield long-range NOE interactions. Methyl grou