A ''Double-WURST'' decoupling sequence is constructed for sitogether with suitable phase cycles, mainly to compensate multaneously decoupling the 13 CO and 13 C a regions in 15 N/ 13 C-doupulse imperfections. ## ble-labeled proteins. It uses frequency-shifted WURST decoupling The early design of
✦ LIBER ✦
URPI: A Utility Program for Planning Selective Protein Double-Labeling with 13C and 15N in NMR Analyses
✍ Scribed by K. Shinagawa; M. Ohya; K. Wakamatsu
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 155 KB
- Volume
- 103
- Category
- Article
- ISSN
- 1064-1866
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HCN, a new 3D NMR technique for stepwise coherence transfer rarely sufficient to establish the mechanism through which from 1 H to 13 C to 15 N and reverse through direct spin couplings it participates in the protein's function. The structure may 1 J CH and 1 J CN , is presented as a method for dete