β Scribed by Ralph S. Santoro; Andrew R. Matz
No coin nor oath required. For personal study only.
1'01. i t . AVO. 10, October 1962 0.092 Gin. of scopolaiiiine hydrobromide into a 100-1111. voluiiietric flask. Dissolve and dilute to volume with p1.I 5.6 buffer.
Intcriiiediate working standard. Dilute 10 nil. of the stock standard to 500 ml. with pH 5.6 buffer.
Working standards. Dilute 12.5-and 25-ml. aliquots of the intermediate working standard to 100 inl. with pH 5.6 buffer. These standards represent 0.140 riig./5 inl. and 0.285 mg./5 ml. belladonna alkaloids.
Apparatus.-Elctt-Suiiinierson photoelectric col-( rimeter. Chromatographic coluiiiii. Approximately 19 i n i n . X 351) niin. with adapter equipped with a stopcock for controlling rate of flow (Ace Glass catalog Nu. 6261). Kiiico rotating cvaporatur. Preparation of the Calibration Curve.-Transfer a 5.(MLinI, aliquot of racli of the two working standards to 125-ml. separators. To a third separator, transfer a 5.00-ml. aliquot o f pH 5.6 buffer to serve as a reagent blank. Add 3.0U inl. of methyl orange reagent from a pipet to each funnel. Extract with 5-15-1111. portions of chloroform. collectiiig the chloruforiii extracts in a 100-ml. volumetric flask containing 15 ml. of 95'7;) ethanol, through a pledget
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