Separation of vitamin K and associated lipids by reversed-phase partition column chromatography

✍ Scribed by John T. Matschiner; William V. Taggart

• Publisher: Elsevier Science
• Year: 1967
• Tongue: English
• Weight: 311 KB
• Volume: 18
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(67)90060-7

✦ Synopsis

Column chromatography

remains the method of choice for the preparative purification of trace neutral lipids because of its capacity to handle appropriate loads. As an adjunct to the class separations achieved on silicic acid, Florisil, or other adsorbents, partition chromatography is valuable because it permits purification based on more subtle structural features such as conformation and homologous patterns. During recent studies on vitamin K in beef liver, we employed a reversed-phase system that permits excellent separation of fat-soluble vitamins and associated lipids and that can be used conveniently for preparative work. The procedure is a modification of several published methods but was most directly adapted from the paper chromatography system of Green and Dam (1) and the partition columns of Wiss and Gloor (2).

Methods

'These studies were supported in part under NIH grant AM 09909. ' Celite 545, Johns-Manville Co., treated with dichlorodimethylsilane (3).