Base composition of DNA from some reptiles
โ Scribed by Olmo, Ettore ;Odierna, Gaetano
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- English
- Weight
- 279 KB
- Volume
- 199
- Category
- Article
- ISSN
- 0022-104X
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โฆ Synopsis
Abstract
The DNA base composition was studied in 9 reptilian species (4 chelonians, 3 saurians, and 2 ophidians) by the thermal denaturation method. This preliminary investigation has brought to light differences in GC percentages and in the shape of melting curves between chelonian DNA and that from saurians and ophidians.
๐ SIMILAR VOLUMES
Equations were determined for the dependency of the melting temperature (TJ of DNA upon the logarithm of the sodium ion concentration, for four DNA samples of widely diflerent base compositions (eoc). The dopee of these T, versus log M equations were found to decrease with increasing Boc of the samp
The dependence of the net hydration of DNA on it,s base composition has been measured by density gradient ultrncentrifugation of three 1)l"u'A's in a series of cesium and lithium salt solutions of different water aativiticxs. 1t;strapolation to zero water activity showed the tkyeridcucc of thc parti