✦ LIBER ✦

On the hydration of DNA. II. Base composition dependence of the net hydration of DNA

✍ Scribed by Mary-Jane B. Tunis; John E. Hearst

Publisher: Wiley (John Wiley & Sons)
Year: 1968
Tongue: English
Weight: 501 KB
Volume: 6
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.1968.360060909

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✦ Synopsis

The dependence of the net hydration of DNA on it,s base composition has been measured by density gradient ultrncentrifugation of three 1)l"u'A's in a series of cesium and lithium salt solutions of different water aativiticxs. 1t;strapolation to zero water activity showed the tkyeridcucc of thc partial specific volume 0 1 1 base composition to be very small for CsDX.4 and zero for 1,iI)XA. At least 907& of the dependence of buoynut density on base composition ran be nccountrd for on the basis of a diffcrcntial hydration, with a mole of adcnine-thymine pairs binding about 2 moles more water than a molc of gunnine-cytosine pairs in CsCI.

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