B Cell Receptor Signaling: Methods and Protocols

✍ Scribed by Chaohong Liu (eds.)

• Publisher: Humana Press
• Year: 2018
• Tongue: English
• Leaves: 262
• Series: Methods in Molecular Biology 1707
• Edition: 1
• Category: Library

✦ Synopsis

This volume looks the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B cell signaling using flow cytometry; confocal microscopy, total internal reflection microscopy and intravital two-photon microscopy; and the methods used to study critical cell components related with B cell activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, B Cell Receptor Signaling: Methods and Protocols is a valuable resource for everyone in the scientific community currently working in the B cell field.

✦ Table of Contents

Front Matter ....Pages i-xi
Activation-Induced Cytidine Deaminase Aided In Vitro Antibody Evolution (Lili An, Chuan Chen, Ruiqi Luo, Yun Zhao, Haiying Hang)....Pages 1-14
Analyzing Mouse B Cell Responses Specific to LCMV Infection (Yaxing Hao, Zhirong Li, Yifei Wang, Xiaobing Liu, Lilin Ye)....Pages 15-38
Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors (Zhiyong Yang, Christopher D. C. Allen)....Pages 39-49
Biophysical Techniques to Study B Cell Activation: Single-Molecule Imaging and Force Measurements (Ivan Rey, David A. Garcia, Brittany A. Wheatley, Wenxia Song, Arpita Upadhyaya)....Pages 51-68
DNA-Based Probes for Measuring Mechanical Forces in Cell-Cell Contacts: Application to B Cell Antigen Extraction from Immune Synapses (Katelyn M. Spillane, Pavel Tolar)....Pages 69-80
Deriving Quantitative Cell Biological Information from Dye-Dilution Lymphocyte Proliferation Experiments (Koushik Roy, Maxim Nikolaievich Shokhirev, Simon Mitchell, Alexander Hoffmann)....Pages 81-94
Flow Cytometry Analysis of mTOR Signaling in Antigen-Specific B Cells (Qizhao Huang, Haoqiang Wang, Lifan Xu, Jianjun Hu, Pengcheng Wang, Yiding Li et al.)....Pages 95-109
Ex Vivo Culture Assay to Measure Human Follicular Helper T (Tfh) Cell-Mediated Human B Cell Proliferation and Differentiation (Xin Gao, Lin Lin, Di Yu)....Pages 111-119
B Cell Receptor Signaling and Compartmentalization by Confocal Microscopy (Anurag R. Mishra, Akanksha Chaturvedi)....Pages 121-129
Imaging the Interactions Between B Cells and Antigen-Presenting Cells (Jia C. Wang, Madison Bolger-Munro, Michael R. Gold)....Pages 131-161
In Vivo Tracking of Particulate Antigen Localization and Recognition by B Lymphocytes at Lymph Nodes (Yolanda R. Carrasco)....Pages 163-169
Study B Cell Antigen Receptor Nano-Scale Organization by In Situ Fab Proximity Ligation Assay (Kathrin Kläsener, Jianying Yang, Michael Reth)....Pages 171-181
Single-Particle Tracking of Cell Surface Proteins (Laabiah Wasim, Bebhinn Treanor)....Pages 183-192
The Use of Intravital Two-Photon and Thick Section Confocal Imaging to Analyze B Lymphocyte Trafficking in Lymph Nodes and Spleen (Chung Park, Il-Young Hwang, John H. Kehrl)....Pages 193-205
Time-Lapse Förster Resonance Energy Transfer Imaging by Confocal Laser Scanning Microscopy for Analyzing Dynamic Molecular Interactions in the Plasma Membrane of B Cells (Hae Won Sohn, Joseph Brzostowski)....Pages 207-224
Understanding of B Cell Receptor Signaling Through a Photo-Activatable Antigen Presentation System (Jing Wang, Zhengpeng Wan, Wanli Liu)....Pages 225-234
Use of Streptolysin O-Induced Membrane Damage as a Method of Studying the Function of Lipid Rafts During B Cell Activation (Heather Miller, Wenxia Song)....Pages 235-241
Visualization and Quantitative Analysis of the Actin Cytoskeleton Upon B Cell Activation (Vid Šuštar, Marika Vainio, Pieta K. Mattila)....Pages 243-257
Back Matter ....Pages 259-262

✦ Subjects

Biochemistry