ErbB receptor signaling : methods and protocols

✍ Scribed by Wang, Zhixiang

Year: 2017
Tongue: English
Leaves: 298
Series: Methods in molecular biology 1652
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This volume contains protocols specifically designed for studying cell signaling mediated by ErbB receptors, all applicable to the study of a broad range of ErbB receptor-mediated signaling from basic research to clinic applications, from cultured cells to various animal models and primary cancer cells from patients. After some overviews, the book continues by covering common protocols for studying various aspects of ErbB receptor-mediated cell signaling, newly-developed methods in biomedical research that are also widely-used in the study of ErbB receptor signaling, epidermal growth factor receptor (EGFR) signaling in Drosophila, as well as protocols for studying ErbB receptor signaling in various animal model systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Detailed and easy-to-follow, ErbB Receptor Signaling: Methods and Protocols serves as an ideal guide for researchers who are studying the cell signaling mediated by ErbB receptors and other receptor tyrosine kinases.

✦ Table of Contents

Front Matter ....Pages i-x
Front Matter ....Pages 1-1
ErbB Receptors and Cancer (Zhixiang Wang)....Pages 3-35
New Insights from Drosophila into the Regulation of EGFR Signaling (Nicholas Harden)....Pages 37-42
C. elegans Vulva Induction: An In Vivo Model to Study Epidermal Growth Factor Receptor Signaling and Trafficking (Kimberley Gauthier, Christian E. Rocheleau)....Pages 43-61
Targeting HER2 in Advanced Breast Cancer (Xiaofu Zhu, Anil Abraham Joy)....Pages 63-77
Front Matter ....Pages 79-79
Methods to Investigate EGFR Ubiquitination (Alexia Conte, Sara Sigismund)....Pages 81-100
Dimerization Assessment of Epithelial Growth Factor Family of Receptor Tyrosine Kinases by Using Cross-Linking Reagent (Hamid Maadi, Babak Nami, Zhixiang Wang)....Pages 101-108
Application of Immunofluorescence Staining to Study ErbB Family of Receptor Tyrosine Kinases (Babak Nami, Zhixiang Wang)....Pages 109-116
Activation of Endosome-Associated Inert EGF Receptor Following Internalization (Yi Wang, Sukhmani Billing, Zhixiang Wang)....Pages 117-126
Two-Pulse Endosomal Stimulation of Receptor Tyrosine Kinases Induces Cell Proliferation (Steven Pennock, Sukhmani Billing, Zhixiang Wang, Yi Wang)....Pages 127-133
Study of EGFR Signaling/Endocytosis by Site-Directed Mutagenesis (Qian Wang, Zhixiang Wang)....Pages 135-143
Using Percoll Gradient Fractionation to Study the Endocytic Trafficking of the EGFR (Julie A. Gosney, Brian P. Ceresa)....Pages 145-158
Analysis of Epidermal Growth Factor Receptor-Induced Cell Motility by Wound Healing Assay (Junfeng Tong, Zhixiang Wang)....Pages 159-163
Front Matter ....Pages 165-165
Cell Cycle Synchronization of HeLa Cells to Assay EGFR Pathway Activation (Ping Wee, Zhixiang Wang)....Pages 167-181
Analysis of Constitutive EGFR Signaling Regulating IRF3 Transcriptional Activity in Cancer Cells (Gao Guo, Ke Gong, Amyn A. Habib)....Pages 183-189
Measurement of Epidermal Growth Factor Receptor-Derived Signals Within Plasma Membrane Clathrin Structures (Stefanie Lucarelli, Ralph Christian Delos Santos, Costin N. Antonescu)....Pages 191-225
Front Matter ....Pages 227-227
Studying Nonproliferative Roles for Egfr Signaling in Tissue Morphogenesis Using Dorsal Closure of the Drosophila Embryo (Bruce Reed, Nicholas Harden)....Pages 229-256
Front Matter ....Pages 257-257
Analysis of Epithelial–Mesenchymal Transition Induced by Overexpression of Twist (Jing-Wen Bai, Yong-Qu Zhang, Yao-Chen Li, Guo-Jun Zhang)....Pages 259-274
Assessment of Specificity of an Adenovirus Targeted to HER3/4 (Sheena H. MacLeod, Kyle G. Potts, Shyambabu Chaurasiya, Mary M. Hitt)....Pages 275-293
Isolation of Human Mesenchymal Stem Cells for Studying ErbB Receptor Signaling (Chao Chen, Hongxing Jiang)....Pages 295-300
Back Matter ....Pages 301-303

✦ Subjects

EPIDERMALER WACHSTUMSFAKTOR (BIOCHEMIE);REZEPTOREN (MOLEKULARBIOLOGIE);INTERZELLULÄRE BIOKOMMUNIKATION;LABORHANDBÜCHER + VERSUCHSANLEITUNGEN (DOKUMENTENTYP);FACTEUR DE CROISSANCE ÉPIDERMIQUE (BIOCHIMIE);EPIDERMAL GROWTH FACTOR (BIOCHEMISTRY);RÉCEPTEURS (BIOLOGIE MOLÉCULAIRE);RECEPTORS (MOLECULAR BIOLOGY);BIOCOMMUNICATION INTERCELLULAIRE;INTERCELLULAR BIOCOMMUNICATION;LABORATORY MANUALS + FIELD MANUALS (DOCUMENT TYPE);GUIDES DE LABORATOIRE (TYPE DE DOCUMENT)

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