โ Scribed by Hiroto Kotaniguchi; Miyako Kawakatsu; Toshimasa Toyo'oka; Kazuhiro Imai
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
It has been shown that by electrochemical oxidation 7-nitrobenzo-2-oxa-1,3-diazole-tryptophan (NBD-T) is converted to fluorophores having the same emission and excitation spectra as those for other NBD-amino acids. NBD-dioxindolylalanine was tentatively assumed to be a main electrochemical oxidation
A high-performance liquid chromatographic method with fluorescence detection was developed for a series of cholinergic channel activators using 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-F) as a fluorescent labeling reagent. This method includes three separate steps: extraction of parent compound
7-Fluoro+nitrobenzo-2-oxa-I .3-diazole is used as a precolumn fluorescent labeling reagent for high-performance liquid chromatography of amino acids. including proline and hydroxyproline. The reaction is run at pH 8.0 at 60ยฐC for 5 min. The fluorophors (Asp, GIL Hyp. Ser, Gly. Thr, Ala, Pro) are se