Colorimetric and Fluorometric Assays of Glutathione Transferase Based on 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole
β Scribed by G. Ricci; A.M. Caccuri; M. Lobello; A. Pastore; F. Piemonte; G. Federici
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 283 KB
- Volume
- 218
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
It has been shown that by electrochemical oxidation 7-nitrobenzo-2-oxa-1,3-diazole-tryptophan (NBD-T) is converted to fluorophores having the same emission and excitation spectra as those for other NBD-amino acids. NBD-dioxindolylalanine was tentatively assumed to be a main electrochemical oxidation
7-Fluoro+nitrobenzo-2-oxa-I .3-diazole is used as a precolumn fluorescent labeling reagent for high-performance liquid chromatography of amino acids. including proline and hydroxyproline. The reaction is run at pH 8.0 at 60Β°C for 5 min. The fluorophors (Asp, GIL Hyp. Ser, Gly. Thr, Ala, Pro) are se
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