Low concentrations of sodium dodecyl sulfate have a dramatic effect on the partial proteolytic products obtained by digesting bovine serum albumin with chymotrypsin or trypin. The effect observed may be important far the inte~retation of peptide maps obtained by the method of D. W. Cleveland et ai.
Automated proteolytic mapping of proteins
โ Scribed by Tim Nadler; Christopher Blackburn; Jonathan Mark; Neal Gordon; Fred E. Regnier; George Vella
- Book ID
- 107786051
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 572 KB
- Volume
- 743
- Category
- Article
- ISSN
- 1873-3778
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๐ SIMILAR VOLUMES
There are many applications of automated techniques in enzyme research (1). Although many methods have been established for manual measurement of proteinases and peptidases (2-6)) the automated measurement of these enzymes has scarcely been attempted, except for recent examples with the Technicon Au
There are many applications of automated techniques in enzyme research (1). For manual measurement of proteolytic enzymes the widest application is found in the measurement of amino acid and peptide products from enzymic hydrolysis of proteins, especially hemoglobin and casein (2, 3). The objective