Automated measurement of proteolytic enzymes

✍ Scribed by A.L. Tappel

• Publisher: Elsevier Science
• Year: 1968
• Tongue: English
• Weight: 412 KB
• Volume: 23
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(68)90238-8

✦ Synopsis

There are many applications of automated techniques in enzyme research (1). For manual measurement of proteolytic enzymes the widest application is found in the measurement of amino acid and peptide products from enzymic hydrolysis of proteins, especially hemoglobin and casein (2, 3). The objective of this research was the development of automated methods which optimize these measurements of products from hydrolysis of proteins. The automated methods developed are especially useful for application to measurements of cathepsin D of lysosomes.

MATERIALS AND METHODS

Enzymes

Cathepsin D. Rat kidney and rat liver lysosomes, prepared by methods similar to those described previously (4, 5), were the source of cathepsins hydrolyzing hemoglobin, mainly cathepsin D.

Papain. Papain (2x tryst.) was obtained from Worthington Biochemicals, Freehold, N. J.

Ficin. Ficin (2x tryst.) was obtained from Nutritional Biochemicals Corp., Los Angeles, Calif.

Substrates