Automated measurement of proteolytic enzymes

✍ Scribed by Y. Ozawa; K. Suzuki; K. Mogi

• Book ID: 102983962
• Publisher: Elsevier Science
• Year: 1971
• Tongue: English
• Weight: 536 KB
• Volume: 43
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(71)90102-3

✦ Synopsis

There are many applications of automated techniques in enzyme research (1). Although many methods have been established for manual measurement of proteinases and peptidases (2-6)) the automated measurement of these enzymes has scarcely been attempted, except for recent examples with the Technicon AutoAnalyzer (7-9). The purpose of the research described here was to develop automated methods for rapid and convenient measurement of proteolytic enzymes.

MATERIALS $KD METHODS

Enzymes

Trypsin. Trypsin (2x crystallized, 50% MgS04) was obtained from Nutritional Biochemicals Corp., Cleveland, Ohio.

Bacillus neutral proteinase. Neutral proteinase from Bacillus s&i&s var. amylosacchariticus Fukumoto (1 X crystallized, 11,000 unitsjmg protein) was purchased from Seikagaku Kogyo Co., Ltd., Tokyo, Japan.

Aspergillus dipeptidase. The enzyme was partially purified from surface culture of Aspergillus sojae X-816 by a modification of Akatsuka's method (10).