Automated method for the determination of vitamin
✍ Scribed by J. M. Mata-Granados; A. Caballo-López; M. D. Luque de Castro; J. M. Quesada
- Publisher
- Springer
- Year
- 2003
- Tongue
- English
- Weight
- 199 KB
- Volume
- 377
- Category
- Article
- ISSN
- 1618-2650
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The first practical method for the determination of hexosamines was worked out by Elson and Morgan (1). Since that time many modifications have been introduced in an attempt to increase the sensitivity and reproducibility of the technique (2) and to decrease the effect of interfering substances (3)
An automated procedure has been described based on the manual method of Hagen and Hagen [(1%2) Canad. J. Biochem. 40, 11291 which will rapidly and reproducibly measure glycerol concentrations. The method was developed primarily for the determination of glycerol in adipose tissue and various incubat