✦ LIBER ✦

Automated analytical systems for drug development studies. V. A system for enzyme kinetic studies

✍ Scribed by J. Zhou; E.C. Shearer; J. Hong; C.M. Riley; R.L. Schowen

Publisher: Elsevier Science
Year: 1996
Tongue: English
Weight: 540 KB
Volume: 14
Category: Article
ISSN: 0731-7085
DOI: 10.1016/0731-7085(96)01792-x

No coin nor oath required. For personal study only.

✦ Synopsis

Two similar automated analytical systems using liquid chromatography (LC) and microdialysis as an on-line sampling technique were applied to studies of enzyme kinetics. 2',3',5'-Triacetyl-6-azauridine (azaribine) with porcine liver esterase (PLE) and N-acetylphenylalanyl-3,5-diiodotyrosine (AcFY') with pepsin were used as model compounds. The microdialysis sampling technique permitted the rapid separation of low molecular weight analytes from macromolecules, thus simultaneously achieving clean-up of the samples and quenching of the reaction. The combination of rapid LC analysis and microdialysis sampling provided selectivity and automation. The systems are rugged and give reproducible results in agreement with those from manual sampling methods.

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