Autogenous growth factor production by reticuloendotheliosis virus-transformed hematopoietic cells

✍ Scribed by Robert F. Garry; Henry R. Bose Jr.

• Book ID: 102876173
• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 775 KB
• Volume: 37
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.240370307

✦ Synopsis

Reticuloendotheliosis virus strain T (REV-T)-transformed cells gave rise spontaneously to variants which secrete a factor that forms a distinct visible ring of precipitation (halo) surrounding colonies grown in soft agar. An M, 15,OOO protein was produced at higher levels by halo variants than by nonhalo-producing cells. An assay designed to detect the formation of precipitates enabled purification of an M, 15 ,OOO protein, p15, from serum-free medium conditioned by the growth of REV-T-transformed hematopoietic cells. Fractions enriched in p15 permitted the growth of REV-T-transformed cells under conditions where they normally failed to proliferate.