## Abstract Chicken embryo fibroblasts and hamster BHK cells transformed by Rous sarcoma virus (RSV) release in their culture media growth factors which enhance markedly anchorageโindependent colony formation in gelified medium, at the restrictive temperature (41ยฐ5 C), of chicken embryo fibroblasts
Reduced binding of epidermal growth factor by avian sarcoma virus-transformed rat cells
โ Scribed by Stuart Decker
- Book ID
- 115757209
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 544 KB
- Volume
- 113
- Category
- Article
- ISSN
- 0006-291X
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A new class of transforming growth factor (TGF), with chemical characteristics differing from previously reported TGFs, was isolated and purified from an avian sarcoma virus-transformed rat cell line, 77N1. Purification steps were simple and consisted of ion-exchange chromatography on diethylaminoet
## Abstract Serumโfree conditioned media was collected from three sarcoma virusโtransformed cell lines and an untransformed cell line. All three virally transformed lines produced and released growth factors into their serumโfree media. The major activity in all cases, whether the cells were transf