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Autoclave method for rapid preparation of bacterial PCR-template DNA

โœ Scribed by Keith E. Simmon; Dewey D. Steadman; Sarah Durkin; Amy Baldwin; Wade H. Jeffrey; Peter Sheridan; Rene Horton; Malcolm S. Shields


Book ID
116749590
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
303 KB
Volume
56
Category
Article
ISSN
0167-7012

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A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed. Bacteria are boiled for 15โ€“40 s and an insoluble clot of genomic DNA and debris is removed by low-speed centrifugation. Plasmids are recovered from the supernatant by isopropanol precipitation and ca