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ATPase activity associated with isolated vacuoles of the crassulacean acid metabolism plantKalanchoë daigremontiana

✍ Scribed by J. Andrew C. Smith; Ernest G. Uribe; Erika Ball; Ulrich Lüttge

Book ID
104755801
Publisher
Springer-Verlag
Year
1984
Tongue
English
Weight
980 KB
Volume
162
Category
Article
ISSN
0032-0935

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✦ Synopsis

A technique is described that allows a relatively rapid and controlled isolation of vacuoles from leaves of the crassulacean acid metabolism (CAM) plant Kalancho~ daigremontiana. The method involves polybase-induced lysis of mesophyllcell protoplasts and isolation of vacuoles on a discontinuous density gradient. ATPase activity is associated with the isolated vacuoles and is not attributable to contamination by cytoplasmic constituents. It is suggested that this ATPase is responsible for the energization of malic-acid accumulation in the vacuole in CAM plants.

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