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ATPase activity associated with vacuoles and tonoplast vesicles isolated from the CAM plant, Kalanchoë daigremontiana

✍ Scribed by Kunio Aoki; Kojiro Nishida


Book ID
110898205
Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
269 KB
Volume
60
Category
Article
ISSN
0031-9317

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ATPase activity associated with isolated
✍ J. Andrew C. Smith; Ernest G. Uribe; Erika Ball; Ulrich Lüttge 📂 Article 📅 1984 🏛 Springer-Verlag 🌐 English ⚖ 980 KB

A technique is described that allows a relatively rapid and controlled isolation of vacuoles from leaves of the crassulacean acid metabolism (CAM) plant Kalancho~ daigremontiana. The method involves polybase-induced lysis of mesophyllcell protoplasts and isolation of vacuoles on a discontinuous dens