ATPase activity associated with isolated
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J. Andrew C. Smith; Ernest G. Uribe; Erika Ball; Ulrich Lüttge
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Article
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1984
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Springer-Verlag
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English
⚖ 980 KB
A technique is described that allows a relatively rapid and controlled isolation of vacuoles from leaves of the crassulacean acid metabolism (CAM) plant Kalancho~ daigremontiana. The method involves polybase-induced lysis of mesophyllcell protoplasts and isolation of vacuoles on a discontinuous dens