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Asymmetric transcription of phage SPP1 DNA

✍ Scribed by Riva, Silvano C.


Book ID
122715314
Publisher
Elsevier Science
Year
1969
Tongue
English
Weight
364 KB
Volume
34
Category
Article
ISSN
0006-291X

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πŸ“œ SIMILAR VOLUMES


SPP1 DNA replicative forms: Growth of ph
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The development of bacteriophages SPP1 and phi 29 has been studied in several B. sutilis mutants defective in host DNA replication, under non permissive conditions. Several gene products, involved in the synthesis of host DNA, are required for phi 29 replication, while SPP1 seems to require only the

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The left part of the SPP1 chromosome can be defined by the presence of dispensable DNA. With this definition, the polarities of the separable H and L strands of SPP1 DNA ar 5'--3' and 3'--5', respectively, from left to right.

The genome of B. subtilis phage SPP1
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41 genes of SPP1 have been delineated by using complementation analyses of 75 conditionally lethal (ts and sus) mutations. The physical locations of these genes on the SPP1 chromosome have been determined by transfection/marker rescue experiments in which restriction endonuclease generated fragments

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SPP1 DNA was cleaved by the restriction endonucleases, BglI, BglII, EcoRI, KpnI, SmaI, and SalI. The molecular weights of the DNA fragments obtained by single enzyme digestion or by consecutive digestion with two enzymes were determined by electron microscopic measurements of contour length and by g