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The genome of B. subtilis phage SPP1

✍ Scribed by Behrens, B. ;Lüder, G. ;Behncke, M. ;Trautner, T. A. ;Ganesan, A. T.

Publisher
Springer
Year
1979
Tongue
English
Weight
608 KB
Volume
175
Category
Article
ISSN
0026-8925

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✦ Synopsis

41 genes of SPP1 have been delineated by using complementation analyses of 75 conditionally lethal (ts and sus) mutations. The physical locations of these genes on the SPP1 chromosome have been determined by transfection/marker rescue experiments in which restriction endonuclease generated fragments of SPP1 DNA were used as donor DNA. The physical order of these fragments has been previously established (Ratcliff et al., 1979).

📜 SIMILAR VOLUMES

The genome of B. subtilis phage SPP1: As
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Phage SPP1 infecting a mutator strain of B. subtilis (BD337) which carries a defective DNA polymerase III is mutagenized. This effect is absent in phages SPO2, SP82G and øe. The results confirm previous observations that SPP1 uses host DNA polymerase III for its DNA synthesis.