41 genes of SPP1 have been delineated by using complementation analyses of 75 conditionally lethal (ts and sus) mutations. The physical locations of these genes on the SPP1 chromosome have been determined by transfection/marker rescue experiments in which restriction endonuclease generated fragments
The genome of B. subtilis phage SPP1: Assignment of 5′–3′-polarity to the complementary strands of SPP1 DNA
✍ Scribed by Morelli, G. ;Montenegro, M. A. ;Hillenbrandt, G. ;Scherzinger, E. ;Trautner, T. A.
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 504 KB
- Volume
- 164
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
The left part of the SPP1 chromosome can be defined by the presence of dispensable DNA. With this definition, the polarities of the separable H and L strands of SPP1 DNA ar 5'--3' and 3'--5', respectively, from left to right.
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SPP1 DNA was cleaved by the restriction endonucleases, BglI, BglII, EcoRI, KpnI, SmaI, and SalI. The molecular weights of the DNA fragments obtained by single enzyme digestion or by consecutive digestion with two enzymes were determined by electron microscopic measurements of contour length and by g