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Assignment of the backbone carbonyl resonances in15N-labelled proteins with13C at natural abundance by a 2D triple-resonance correlation technique

✍ Scribed by Søren M. Kristensen; Morten Dahl Sørensen; Jens J. Led


Book ID
118287238
Publisher
Springer Netherlands
Year
1995
Tongue
English
Weight
361 KB
Volume
5
Category
Article
ISSN
0925-2738

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A 3D triple resonance experiment has been designed to provide intraresidual and sequential correlations between amide nitrogens and c~-carbons in uniformly 13C/15N-labeled proteins. In-phase 13C~ magnetization is transferred to the aliphatic side-chain protons via the side-chain carbons using a CC-T