We thank Claude Klee for help and encouragement in our study of calcineurin B, Hao Ren for protein expression, and Marius Clore and Dennis Torchia for useful discussions. This work was supported by the AIDS Targeted Anti-Viral Program of the Office of the Director of the National Institutes of Healt
A 4D HCC(CO)NNH experiment for the correlation of aliphatic side-chain and backbone resonances in13C/15N-labelled proteins
✍ Scribed by Robin T. Clowes; Wayne Boucher; Colin H. Hardman; Peter J. Domaille; Ernest D. Laue
- Publisher
- Springer Netherlands
- Year
- 1993
- Tongue
- English
- Weight
- 378 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0925-2738
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A 3D triple resonance experiment has been designed to provide intraresidual and sequential correlations between amide nitrogens and c~-carbons in uniformly 13C/15N-labeled proteins. In-phase 13C~ magnetization is transferred to the aliphatic side-chain protons via the side-chain carbons using a CC-T
Recently, a novel three-dimensional, triple-resonance ex-respect to the nitrogen, which then evolves into in-phase periment was described which correlates intraresidue 13 CO, carbonyl magnetization again. Evolution under the 1 J CaCO 13
HCN, a new 3D NMR technique for stepwise coherence transfer rarely sufficient to establish the mechanism through which from 1 H to 13 C to 15 N and reverse through direct spin couplings it participates in the protein's function. The structure may 1 J CH and 1 J CN , is presented as a method for dete