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Assessment of smoking-induced DNA damage in lymphocytes of smoking mothers of newborn infants using the alkaline single-cell gel electrophoresis technique

✍ Scribed by S. Şardaş; D. Walker; D. Akyol; A.E. Karakaya


Book ID
113253010
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
447 KB
Volume
335
Category
Article
ISSN
0165-1161

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The alkaline single cell gel electrophoresis (SCGE) or "comet" assay under alkaline conditions was used to measure DNA damage in the liver cells of B6C3F1 male mice exposed to 2,4-dimethylaniline and 2,4,6-trimethylaniline. Cells embedded in agarose were lysed, subjected briefly to an electric field