Assessment of radiation-induced DNA damage caused by the incorporation of 99mTc-radiopharmaceuticals in murine lymphocytes using single cell gel electrophoresis
✍ Scribed by Martha Pedraza-López; Guillermina Ferro-Flores; Ma.Teresa Mendiola-Cruz; Pedro Morales-Ramı́rez
- Book ID
- 117767966
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 72 KB
- Volume
- 465
- Category
- Article
- ISSN
- 1383-5718
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📜 SIMILAR VOLUMES
Human lymphocytes from a cell culture were irradiated with laser pulses of an Xe-Cl excimer laser (308 nm) or a dye laser at wavelengths from 312 to 640 nm. After conversion of photoinduced DNA damage into DNA strand breaks the "comet assay" (single-cell gel electrophoresis) was used to determine th
The alkaline single cell gel electrophoresis (SCGE) or "comet" assay under alkaline conditions was used to measure DNA damage in the liver cells of B6C3F1 male mice exposed to 2,4-dimethylaniline and 2,4,6-trimethylaniline. Cells embedded in agarose were lysed, subjected briefly to an electric field