In this work the maximal operational hydrodynamic conditions (agitation and aeration rate) that cause no adverse effect in Sf-9 cells growth in SF900II serumfree medium were determined. Shear stresses higher than 1 N m -2 and aeration rates higher than 0.04 vvm affect cell growth and when these cond
Assembly of JC virus-like particles in COS7 cells
โ Scribed by Shishido, Yukiko; Nukuzuma, Soichi; Mukaigawa, Jun; Morikawa, Shigeru; Yasui, Kotaro; Nagashima, Kazuo
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 518 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0146-6615
No coin nor oath required. For personal study only.
โฆ Synopsis
JC virus lacks an appropriate cell line to support
1985
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## Abstract Oncornavirusโlike particles observed and purified from HeLa cell culture supernates have a density in sucrose of 1.16โ1.17 g/cm^3^. They contain DNA polymerase and ribonuclease activities with properties characteristic of reverse transcriptase and ribonuclease H of known oncornaviruses.
## Abstract An oncornavirusโlike agent has been described in HeLa cells of human origin (Gelderblom __et al.__, 1974). The investigation of its protein constituents revealed a pattern similar to but different from that of known oncornaviruses. Six polypeptides with molecular weights (mw) between 12