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Assays for cholesterol 7α-hydroxylase and 12α-hydroxylase using high performance liquid chromatography

✍ Scribed by Mitsuhide Noshiro; Hiroko Ishida; Shin-ichi Hayashi; Kyuichiro Okuda

Book ID
118979198
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
572 KB
Volume
45
Category
Article
ISSN
0039-128X

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✍ Hiromitsu Yokohama; Issei Ohtsuka; Hiroyuki Shiojiri; Kouich Katayama; Seiji Ish 📂 Article 📅 1986 🏛 Elsevier Science 🌐 English ⚖ 379 KB

A high-performance liquid chromatographic method has been developed for the measurement of cholesterol 7cy-hydroxylase activity in liver microsomes. 7ol-Hydroxycholesterol generated from endogenous choleserol was derivatized with anthroyl l-c&o&rile, chromatogmphed on a revere phase column, and dete