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A high-performance liquid chromatographic method for the assay of cholesterol 7α-hydroxylase activity

✍ Scribed by Hiromitsu Yokohama; Issei Ohtsuka; Hiroyuki Shiojiri; Kouich Katayama; Seiji Ishikawa

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
379 KB
Volume
157
Category
Article
ISSN
0003-2697

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✦ Synopsis

A high-performance liquid chromatographic method has been developed for the measurement of cholesterol 7cy-hydroxylase activity in liver microsomes. 7ol-Hydroxycholesterol generated from endogenous choleserol was derivatized with anthroyl l-c&o&rile, chromatogmphed on a revere phase column, and detected fluorometrically. The detection limit of 7a-hydroxycholesterol was I r&tube. The cholesterol 7o-hydroxylase activity in rat liver microsomes was assayed by this method, and the effects of some detergents and of the addition of exogenous cholesterol together with detergents on the enzyme activity were investigated. The endogenous 7~ and 'la-hydroxycholesterol could be also measured by this method. Q 1986 academic p, IW.

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