The activity of peptidylarginine deiminase (PAD) has generally been assayed by a colorimetric method using N-benzoyl-L-arginine ethyl ester (BAEE) and Nbenzoyl-L-arginine (Bz-L-Arg) as the substrates. The widespread occurrence of citrulline and urea in tissues makes use of this method difficult, esp
A sensitive method for determination of biphenyl hydroxylase activities using high-performance liquid chromatography
β Scribed by Y. Yamazoe; T. Kamataki; R. Kato
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 393 KB
- Volume
- 111
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A new method for measurement of myosin ATPase activity has been developed utilizing reversed-phase high-performance liquid chromatography (HPLC), which detects as low as 0.05 nmol of ADP hydrolyzed from ATP. After termination of the ATPase reaction by addition of perchloric acid, the hydrolysate ADP
A high-performance liquid chromatographic method has been developed for the measurement of cholesterol 7cy-hydroxylase activity in liver microsomes. 7ol-Hydroxycholesterol generated from endogenous choleserol was derivatized with anthroyl l-c&o&rile, chromatogmphed on a revere phase column, and dete
Coupled fi-cyclodextrin bonded-phase and reverse-phase high-performance liquid chromatography with fluorescence detection has been employed to detect the major hydroxylated metabolites of biphenyl following in virro incubation with hepatic 9000~ supernatant. The method requires only 0.3 mg of prote