Assaying Sterile Concentrated Milk for Native Proteolytic Enzymes
โ Scribed by Nakai, S.; Wilson, H.K.; Herreid, E.O.
- Book ID
- 122019228
- Publisher
- American Dairy Science Association
- Year
- 1964
- Tongue
- English
- Weight
- 328 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0022-0302
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๐ SIMILAR VOLUMES
A method is described which permits the assay of proteolytic enzyme activity on protein substrates without precipitation or filtration steps, utilizing a fluorescent reagent which is specific for primary amines. The assay is about 100 times more sensitive than the Lowry method, much faster and less
A simple inexpensive sensitive protease assay was developed using soluble fluorescein isothiocyanate (FITC)-labeled casein. Casein was reacted with FITC to form the fluorescein thiocarbamoyl derivative. This substrate is cleaved by trypsin, chymotrypsin, elastase, subtilisin, and thermolysin in a li