Assay of Collagenase Activity by a Rapid, Sensitive, and Specific Method
✍ Scribed by Lasse Ryhänen; Sirpa Rantala-Ryhänen; Elaine M.L. Tan; Jouni Uitto
- Book ID
- 114178961
- Publisher
- Elsevier
- Year
- 1982
- Weight
- 955 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0174-173X
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📜 SIMILAR VOLUMES
## Abstract A fast and specific method for the detection of collagenase by electrophoresis is described. The method avoids inclusion of the substrate in the resolving gel and can detect nanogram levels of the enzyme.
A simplified assay method is described for the determination of protein kinase activity. Enzymatic activity is followed by measuring the incorporation of 32P from the terminal phosphoryl group of nucleoside triphosphates into protein substrate. Separation of the resulting 32P-labeled phosphoprotein