✦ LIBER ✦

Assay of cardiolipin peroxidation by high-performance liquid chromatography

✍ Scribed by N.L. Parinandi; B.K. Weis; H.H.O. Schmid

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 371 KB
Volume: 49
Category: Article
ISSN: 0009-3084
DOI: 10.1016/0009-3084(88)90009-6

No coin nor oath required. For personal study only.

✦ Synopsis

Commercial preparations of bovine cardiolipin (diphosphatidylglyceroi) in chloroform solution contain substantial amounts of oxidation products. These oxidized derivatives, characterized by the presence of varying amounts of hydroperoxides and conjugated diem, can be separated from unoxidized cardiofipin by normal phase high-performance liquid chromatography (HPLC) using UV detection. When purified cardiolipin is subjected to autoxidation in aqueous media, oxidation products of similar I-IPLC properties are produced. Storage of cardiolipin in chloroform induces both autoxidation and hydrolysis whereas storage in ethanol and other solvents does not. It is recommended not to use chloroform for the long-term storage of cardiofipin.

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