Galactosyltransferase catalyzes transfer of galactose from UDP-galactose to glucose or N-acetylglucosamine with resultant formation of galactosides and UDP. In this new assay galactosyltransferase activity is measured by determining UDP by isocratic high-performance liquid chromatography on an amino
Assay of ornithine aminotransferase by high-performance liquid chromatography
β Scribed by James J. O'Donnell; Robert P. Sandman; Susan R. Martin
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 253 KB
- Volume
- 90
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
Omithine aminotransferase has been measured previously with a spectrophotometric assay and with a radioactive assay. We report here an isocratic reverse phase high-performance liquid chromatography assay which measures A'pyrroline-S-carboxylic acid, the reaction product. This assay offers the advantages of sensitivity and convenience.
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