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Assay of galactosyltransferase by high-performance liquid chromatography

✍ Scribed by A.Jeanne Hymes; Franklin Mullinax

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
369 KB
Volume
139
Category
Article
ISSN
0003-2697

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✦ Synopsis

Galactosyltransferase catalyzes transfer of galactose from UDP-galactose to glucose or N-acetylglucosamine with resultant formation of galactosides and UDP. In this new assay galactosyltransferase activity is measured by determining UDP by isocratic high-performance liquid chromatography on an amino-bonded column monitored spectrophotometrically. Concurrently, unreacted UDP-galactose and breakdown products arising from UDP-galactose (UMP and uridine) are also determined. The new technique does not require radioactive substrates, permits usage of saturating concentrations of UDP-galactose, and provides monitoring of side reactions.

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