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Assay of 3-hydroxy-3-methylglutaryl CoA reductase activity using anionic-exchange column chromatography

✍ Scribed by K.K. Ong; H.T. Khor; D.T.S. Tan


Book ID
107718976
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
410 KB
Volume
196
Category
Article
ISSN
0003-2697

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A new method for the evaluation of 3-hydroxy-3-methylglutaryl-CoA reductase activity is described, based on the multiple-selected ion monitoring of the amount of mevalonate formed in incubations of 3-hydroxy-3-methylghrtaryl-CoA with microsomal proteins. Analysis is carried out on crude extracts usi