Activation of purified 3-hydroxy-3-methylglutaryl-CoA reductase by phospholipids
โ Scribed by Charles B. Berde; Renu A. Heller; Robert D. Simoni
- Book ID
- 115737407
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 873 KB
- Volume
- 488
- Category
- Article
- ISSN
- 0005-2760
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A new method for the evaluation of 3-hydroxy-3-methylglutaryl-CoA reductase activity is described, based on the multiple-selected ion monitoring of the amount of mevalonate formed in incubations of 3-hydroxy-3-methylghrtaryl-CoA with microsomal proteins. Analysis is carried out on crude extracts usi
Addition of hemoglobin, methemoglobin, hemin or hematin in the assay mixture of rat liver 3-hydroxy-3-methylglutaryl CoA (HMGCoA) reductase inhibited the activity of the enzyme. The inhibition by hemin was rapid, without any apparent dependence on time of preincubation. At 20 microM hemin, a maximum