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Evaluation of 3-hydroxy-3-methylglutaryl-CoA reductase activity by multiple-selected ion monitoring

✍ Scribed by G. Cighetti; E. Santaniello; G. Galli

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
443 KB
Volume
110
Category
Article
ISSN
0003-2697

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✦ Synopsis

A new method for the evaluation of 3-hydroxy-3-methylglutaryl-CoA reductase activity is described, based on the multiple-selected ion monitoring of the amount of mevalonate formed in incubations of 3-hydroxy-3-methylghrtaryl-CoA with microsomal proteins. Analysis is carried out on crude extracts using deuterated mevalonic acid la&one as internal standard. The sensitivity of the technique allows the quantitative evaluation of mevalonate in microassays (100 pg microsomal protein) of the enzyme activity at the minimum value of the diurnal rhythm.

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