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Assay for S-adenosylmethionine: Methionine methyltransferase

✍ Scribed by Betty D. Allamong; Lila Abrahamson

Publisher: Elsevier Science
Year: 1973
Tongue: English
Weight: 454 KB
Volume: 53
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(73)90080-8

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✦ Synopsis

methionixle methyltransferase in phosphate buffer extracts has been developed. This enzyme catalyzes the biosynthesis of S-methylmethionine from methioninc and S-adenosylmethionine.

The radioactively labeled product. S-methplmethionine, is first separated from the radioactively labeled substrate, L-methionine, by means of ion-exchange chromatography.

Once separated thuslp, the amount present can then be directly determined by the use of a liquid scintillation spectrometer.

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