## Abstract Two pools of seven brains each from adult Swiss‐Webster mice were homogenized, and supernatants were collected for bioassay. PC‐12 cells were placed in a bioassay plate at time zero, at a concentration of 10^4^ cells per well, and primed for 48 hours in a medium containing 50 ng/ml of m
Artifactual presence of β-nerve growth factor in adult mouse brain
✍ Scribed by Michael B. Rosenberg; Mark H. Grossman; Xandra O. Breakefield
- Book ID
- 118869619
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 465 KB
- Volume
- 295
- Category
- Article
- ISSN
- 0006-8993
No coin nor oath required. For personal study only.
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The complete mouse prepro-nerve growth factor (NGF) DNA was fused to the carboxyl terminus of the 6-galactosidase (lac-z) gene of Escherichia coli. Similarly, a genomic fragment encoding the human NGF comprising codons 11 to 106 (from a total of 118) was fused to the fifth codon of the amino terminu
## Abstract The nerve growth factor protein (NGF) isolated from mouse submaxillary gland has been shown to be necessary to the development of vertebrate sensory and sympathetic ganglia. Also, wide ranging alterations in the levels of NGF in the peripheral circulation of humans suffering from a vari