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Approaches to the development of spectrophotometric reaction-rate methods by use of immobilized enzymes in continuous-flow systems

✍ Scribed by J.M. Fernández-Romero; M.D. Luque de Castro; M. Valcárcel

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
710 KB
Volume
274
Category
Article
ISSN
0003-2670

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✦ Synopsis

Three contmuous-flow approaches to the development of spectrophotometnc reactton-rate methods based on the use of unmobdlzed enzymes are proposed They are based on the use of an optlcal blosensor that integrates reaction and detectron m the flow cell, or reversing the flow duectlon and passmg the sample plug lteratwely through the enzyme reactor and the detector, or a cychc system that allows the sample plug to bc trapped m a clrcutt that includes the enzyme reactor and the detector A parameter was defined to compare the features of the methods developed by using these three approaches

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