By using the non-isotopic single-strand conformation polymorphism (SSCP) technique to analyse products of the polymerase chain reaction (PCR), we detected a 561-adenine to cytosine substitution resulting in an amino acid exchange from serine to arginine at position 128 of the E-selectin gene. If thi
Application of PCR to human gene detection
β Scribed by Y-M.Dennis Lo; Alexander F. Markham
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 438 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0958-1669
No coin nor oath required. For personal study only.
β¦ Synopsis
The advent of the polymerase chain reaction has stimulated the development of a number of rapid methods for characterizing human genes. In addition, the unprecedented level of sensitivity offered by some of these methods may prove to be of great value in the detection of minority cell populations. Over the past year, technical developments have been made in this area.
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