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Polymorphism in the human E-selectin gene detected by PCR-SSCP

✍ Scribed by Katrin Wenzel; Rita Hanke; Astrid Speer

Publisher
Springer
Year
1994
Tongue
English
Weight
160 KB
Volume
94
Category
Article
ISSN
0340-6717

No coin nor oath required. For personal study only.

✦ Synopsis

By using the non-isotopic single-strand conformation polymorphism (SSCP) technique to analyse products of the polymerase chain reaction (PCR), we detected a 561-adenine to cytosine substitution resulting in an amino acid exchange from serine to arginine at position 128 of the E-selectin gene. If this amino acid substitution has an effect on the adhesion of blood cells to the endothelium, the polymorphism could be of interest with respect to association studies in a number of pathological conditions, such as cardiovascular diseases.

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